405 LS microplate washer

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The 405™ LS Microplate Washer is the fifth generation BioTek washer, known for superior performance and reliability. Built-in automated buffer switching and "quick-change" manifold designs provide improved ease of use and efficiency when washing 96 and 384 well microplates.

Washer maintenance is critical for excellent assay performance, and the 405 LS now offers the new patented* Verify™ Technology for clog detection and patented Ultrasonic Advantage™ for automated manifold cleaning, making the 405 LS a truly 'self-maintaining' system.

The 405 LS has a simple user interface for creation and execution of wash, dispense, aspirate and maintenance protocols within the extensive onboard software. For use under computer control, BioTek’s LHC™ Software is available in standard and 21 CFR Part 11 compliant versions. The optional BioStack™ is available for walk-away automation of up to 50 microplates.

Cell-based assays are easily processed with the 405 Select LS, with its angled dispense tubes, extensive aspirate and dispense rates and plate position controls providing optimal conditions for washing even fragile cell layers. Magnetic separation and vacuum filtration modules are available for washing magnetic and polystyrene beads used in a growing number of multiplex assays and bead-based ELISAs, such as those developed on the Luminex® xMAP® technology platform. The vacuum filtration feature automates the wash steps of filter bottom plates with five different vacuum levels for optimal performance of filter pore sizes ranging from 0.45 µm to 1.2 µm. The 405 LS is Luminex® xMAP® approved.

A range of configurations for different microplate formats and assay requirements is available for microplate washing, each designed for optimal performance for your plate type and application.

* EP 1 637 887

US Federal Government customers: This product is available on GSA contract. Contact BioTek Customer Care with questions or submit a Quote Request Form to receive pricing and information on specific models (please note GSA Contract in the Comments field).

xMAP® is a registered trademark of Luminex® Corporation.
FLIPR® is a registered trademark of Molecular Devices®

Hardware:

Patented built-in Verify™ sensor controlled through Liquid Handling Control™ (LHC) pc software, automatically checks dispense/aspirate performance and reports clogged tubes:

Accommodates 96- and 384-well microplates
2x24 character backlit LCD display and membrane keypad user interface
Patented built-in Ultrasonic Advantage™ automatically cleans clogged tubes
Automated internal 4-buffer switching
Internal positive displacement pump eliminates use of troublesome pressure delivery systems and allows use of any size dispense reservoir
Built-in priming trough allows automatic manifold priming and rinsing during both operation and maintenance modes and eliminates need for blank plates when used in automated robotic systems
Quick change manifold design
BioStack and 3rd party automation compatible
All washers biomagnetic separation ready (accessory magnets sold separately)
All washers vacuum filtration ready (accessory filtration modules sold separately)

405 Select LS

96-/384-well washing with patented 96-tube Dual-Action™ manifold
Cell washing is optimized with two low-flow rates and 20° angled dispense tubes ideal for washing loosely adherent cell monolayers

405 HT LS

Fast 384-well washing with patented 192-tube Dual-Action™ manifold for independent dispense and aspiration control for precise overfill washing and overflow protection:
Superior tube-in-tube aspiration manifold design for 384-well washing with buffers not containing surfactant
96-well washing ready with optional 96-tube Dual-Action manifold

405 LS

405 LS models automate basic 96-well dispensing and aspiration

Software:

Quick menu allows fluid paths to be easily primed, wash process started and manifold cleaned
Predefined protocols included for out-of-box washing
Fully customizable protocol parameters for individualized wash requirements - gentle cell-washing to vigorous wash for high-binding assays
AutoPrime and auto maintenance routines
Additional software flexibility and 21CFR Part 11 compliance with LHC™ Software for method development, downloading and washing plates
Run protocols created onboard or downloaded from LHC Software

Part #	384-well	96-well	Optimized Cell Washing	4-Buffer Switching	Ultrasonic Advantage	Verify Technology	Biomagnetic Separation**	Vacuum Filtration**
405LSHT	•	*			•		•	•
405LSHTV	•	*		•	•		•	•
405LSU	•	•	•				•	•
405LSUV	•	•	•	•			•	•
405LSUS	•	•	•		•		•	•
405LSUSQ	•	•	•		•	•	•	•
405LSUVS	•	•	•	•	•		•	•
405LSUVSQ	•	•	•	•	•	•	•	•
405LSR		•					•	•
405LSRV		•		•			•	•
405LSRS		•			•		•	•
405LSRSQ		•			•	•	•	•
405LSRVS		•		•	•		•	•
405LSRVSQ		•		•	•	•	•	•

* 96-well washing available with optional manifold PN 1170010, sold separately
** With available accessory module sold separately

405™ LS Microplate Washer Configurations
	Feature	Design
HT	Faster 384-well microplate washing	192-tube (16 x 12) manifold Dual-Action™ HT Includes Ultrasonic Advantage
V	Automated Internal 4-Buffer Switching
S	Ultrasonic Advantage™	Built-in ultrasonic cleaner for manifold self-maintenace
U	96-/384-well microplate washing	96-tube (8 x 12) manifold Dual-Action™
R	96-well microplate washing	96-tube (8 x 12) manifold
Q	Verify™ Technology	Built-in clog detection for dispense and aspirate tubes

General
Microplate Types	96- and 384-well Low profile and standard height Solid and filter bottom (option)
Onboard Software	Create, edit or run multiple protocols
Software (pc control)	LHC2 pc software LHC2 Secure for 21 CFR Part 11 compliance (option) SiLA Compliant driver (option)
Separation	Biomagnetic separation, vacuum filtration (optional)
Shaking	Programmable up to 60 minutes Slow, medium, fast or variable
Soaking	Programmable up to 60 minutes
Ultrasonic Advantage™	Yes (option)
Verify™ clog detection	Yes (option)
Automation	BioStack and 3rd party automation compatible BioSpa 8 Automated Incubator compatible
Washing
Manifold Types	96-well washing: 96-tube manifold - straight dispense tubes 96- and 384-well washing: 96-tube Dual-Action manifold - 20° angled dispense tubes 384-well washing (fast): 192-tube Dual-Action manifold - 7° angled dispense tubes
Volume Range	25 to 3,000 µL/well, in 1 µL increments
Wash Cycles	1 - 250
Buffer/reagent selection	Auto switching (internal) for up to 4 buffers (option)
Supply Bottle	4 L or 10 L (optional)
Dispense Precision	<3% CV: 300 µL/well (96-well washing) <4% CV: 80 µL/well (384-well washing)
Residual Volume	<2 µL/well (96- & 384-well plates) 96-tube manifold for 96 wells; 192-tube for 384 wells
Wash Speed	96-wells, 300 µL/well, 3 cycles; <30 seconds 384-wells,100 µL/well, 3 cycles: <80 seconds 384-wells, 400 µL/well, 1 cycle: <20 seconds
Flow Rates	High flow to low flow Optimized rates for cell assays
Sterilization	Chemical
Vacuum Filtration	Selectable: Approximate final at 30 seconds: Lowest: -38 mmHg Low: -113 mmHg Medium: -200 mmHg High: -390 mmHg Highest: -506 mmHg Vacuum filtration time range: 5 to 999 seconds
Physical Characteristics
Power	100 - 240 Volts AC. 50/60 Hz
Dimensions	14" W x 17" D x 10" H (35.6 x 43.2 x 25.4 cm)
Weight	With internal buffer switching - 36 lbs (16.5 kg) Without - 30 lbs (13.5 kg)
Regulatory
Regulatory	CE and TUV marked. ROHS compliant. In Vitro Diagnostic use models are available.

Specifications are subject to change. Performance values represent the average observed factory test values.

Optional

BioSpa 8 Automated Incubator

BioSpa 8 is an automated incubator linking BioTek readers or imagers together with washers and dispensers for full workflow automation of up to 8 microplates. Temperature, CO₂/O₂ and humidity levels are controlled and monitored through the BioSpa software to maintain an ideal environment for cell cultures during all experimental stages.

BioStack Microplate Stacker

BioStack™ is a compact and versatile microplate stacker compatible with BioTek’s washers, dispensers, detectors and imaging systems. BioStack offers rapid plate exchange speeds to increase throughput and enhance productivity, accommodating assay workflows both with and without plate lids.

Liquid Handling Control™ Software

Software is a powerful yet flexible interface for use with BioTek’s liquid handlers including the 405™ Microplate Washer, the ELx405™ Select Deep Well Washer, the EL406™ Washer Dispenser and the MultiFlo™ Dispenser. New LHC Secure offers compliance to the FDA directive 21 CFR Part 11 or whenever additional security is required.

Standard (Included)
Operators Manual
MANUAL	Operator's manuals can be obtained via the Customer Resource Center

Optional
BioStack Integration Kits
7310040	BioStack Integration Kit for 405™ TS and 405™ LS
Bottles & Tubing
1173000	Dispense Bottle - with Filter, 10L
1173002	Cap for Dispense Bottle - with Filter, 10L
1173003	Cap for Dispense Bottle - with Filter, 4L
1173031	Dispense Bottle – with Filter, 4L
7100531	Cap for Waste Bottle, 4L (Also fits 10L or 20L)
7100533	Waste Tubing Set
7100534	Waste Bottle, 4L
7100537	Dispense Tubing Set – 4 Buffers
7100538	Dispense Tubing Set –1 Buffer
7100542	Waste Bottle with Level Sensing, 4L
7100543S	Waste Set with Level Sensing, 4L Bottles
7100544	Cap for Waste Bottle with Level Sensing, 4L
7100556	Waste Bottle and Tubing, 20L
7100557	Waste Bottle and Tubing, 10L
Cables
75110	Serial cable, 9-pin male to 9-pin male
Dispense/Waste Systems
1170528S	Complete Dispense System, 10L Bottles
1170529S	Complete Dispense System, 4L Bottles
1170530	Complete Dispense/Waste System 115V/230V, 4L Bottles
1170531S	Complete High Flow Waste System 115V/230V, 10L Bottles
1170532	Complete Dispense/High Flow Waste System 115V/230V, 4L Bottles
1170533	Complete Dispense/High Flow Waste System 115V/230V, 10L Bottles
1170534S	Complete Waste System 115V/230V, 10L Bottles
1170535	Complete Dispense/Waste System 115V/230V, 10L Bottles
1170554	Complete Dispense/Waste System 115V/230V, 20 L Bottles
1170555S	Complete Waste System 115V/230V, 20L bottles
1170556	Complete Dispense/High Flow Waste System 115V/230V, 20L Bottles
1170557S	Complete High Flow Waste System 115V/230V, 20L Bottles
7100582S	Vacuum Set with Level Sense Assembly, 10L
7100746S	Complete Waste System 115V/230V, 4L Bottles
7100753S	Complete High Flow Waste System 115V/230V, 4L Bottles
7100754	High Flow Vacuum Pump 115/230V
7103034	Standard Vacuum Pump 115V/230V
Filters
01310	Filter for Dispense Bottle
48294	Filter for Waste Tubing
Manifolds
1170010	96-Tube Dual-Action Manifold Kit, with 7° dispense tubes and tube-in-tube aspiration manifold kit for 405 HT TS and 405 HT LS models only
1172046S	96-tube Dual-Action™ Tube-in-Tube Aspiration Manifold
7102068S	96-tube Dual-Action™ 7° Dispense Manifold
Miscellaneous
01113	Silencing Muffler for Standard Vacuum Pump
1170008	96-well Vacuum Filtration Kit
1170009	96-/384-well Vacuum Filtration Kit
1170013	15' extension kit for use with robotic system integrations of 405 TS / LS
4030551	Vacuum Gauge/Regulator
7100025S	Stylus Kit for any 405™/ELx405™ Select Deep Well manifold
7102215	384-well Ring Magnet
7102216	96-well Ring Magnet
7103016	96-well Flat Magnet
7103017	384-well Flat Magnet
7210515	3-Instrument Rack – for third party robotic system integration
Product Qualification Packages
1170543	405 LS Qualification Package - IQ/OQ/PQ
Test Plates & Reagents
1240001	Replacement 405 Verify test plate kit
Waste Systems
1170555S	Complete Waste System 115V/230V, 20L Bottles
7100764S	Waste set with Level Sensing, 20L Bottles