405 LS microplate washer

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The 405™ LS Microplate Washer is the fifth generation BioTek washer, known for superior performance and reliability. Built-in automated buffer switching and "quick-change" manifold designs provide improved ease of use and efficiency when washing 96 and 384 well microplates.

Washer maintenance is critical for excellent assay performance, and the 405 LS now offers the new patented* Verify™ Technology for clog detection and patented Ultrasonic Advantage™ for automated manifold cleaning, making the 405 LS a truly 'self-maintaining' system.

The 405 LS has a simple user interface for creation and execution of wash, dispense, aspirate and maintenance protocols within the extensive onboard software. For use under computer control, BioTek’s LHC™ Software is available in standard and 21 CFR Part 11 compliant versions. The optional BioStack™ is available for walk-away automation of up to 50 microplates.

Cell-based assays are easily processed with the 405 Select LS, with its angled dispense tubes, extensive aspirate and dispense rates and plate position controls providing optimal conditions for washing even fragile cell layers.  Magnetic separation and vacuum filtration modules are available for washing magnetic and polystyrene beads used in a growing number of multiplex assays and bead-based ELISAs, such as those developed on the Luminex® xMAP® technology platform. The vacuum filtration feature automates the wash steps of filter bottom plates with five different vacuum levels for optimal performance of filter pore sizes ranging from 0.45 µm to 1.2 µm. The 405 LS is Luminex® xMAP® approved.

A range of configurations for different microplate formats and assay requirements is available for microplate washing, each designed for optimal performance for your plate type and application.

* EP 1 637 887

 

US Federal Government customers: This product is available on GSA contract. Contact BioTek Customer Care with questions or submit a Quote Request Form to receive pricing and information on specific models (please note GSA Contract in the Comments field).

xMAP® is a registered trademark of Luminex® Corporation.
FLIPR® is a registered trademark of Molecular Devices®

Hardware:

  • Patented built-in Verify™ sensor controlled through Liquid Handling Control™ (LHC) pc software, automatically checks dispense/aspirate performance and reports clogged tubes:

  • Accommodates 96- and 384-well microplates
  • 2x24 character backlit LCD display and membrane keypad user interface
  • Patented built-in Ultrasonic Advantage™ automatically cleans clogged tubes
  • Automated internal 4-buffer switching
  • Internal positive displacement pump eliminates use of troublesome pressure delivery systems and allows use of any size dispense reservoir
  • Built-in priming trough allows automatic manifold priming and rinsing during both operation and maintenance modes and eliminates need for blank plates when used in automated robotic systems
  • Quick change manifold design
  • BioStack and 3rd party automation compatible
  • All washers biomagnetic separation ready (accessory magnets sold separately)
  • All washers vacuum filtration ready (accessory filtration modules sold separately)

405 Select LS

  • 96-/384-well washing with patented 96-tube Dual-Action™ manifold
  • Cell washing is optimized with two low-flow rates and 20° angled dispense tubes ideal for washing loosely adherent cell monolayers

405 HT LS

  • Fast 384-well washing with patented 192-tube Dual-Action™ manifold for independent dispense and aspiration control for precise overfill washing and overflow protection:
  • Superior tube-in-tube aspiration manifold design for 384-well washing with buffers not containing surfactant
  • 96-well washing ready with optional 96-tube Dual-Action manifold

405 LS

  • 405 LS models automate basic 96-well dispensing and aspiration

Software:

  • Quick menu allows fluid paths to be easily primed, wash process started and manifold cleaned
  • Predefined protocols included for out-of-box washing
  • Fully customizable protocol parameters for individualized wash requirements - gentle cell-washing to vigorous wash for high-binding assays
  • AutoPrime and auto maintenance routines
  • Additional software flexibility and 21CFR Part 11 compliance with LHC™ Software for method development, downloading and washing plates
  • Run protocols created onboard or downloaded from LHC Software
Part #
384-well
96-well
Optimized Cell Washing
4-Buffer Switching
Biomagnetic Separation**
Vacuum Filtration**
405LSHT
 *
   
 
405LSHTV
 *
 
 
405LSU
     
405LSUV
 •
 •
 
 
405LSUS
 
 
405LSUSQ
 •
 •
 
405LSUVS
 
405LSUVSQ
 •
 •
405LSR
 
 
 
   
405LSRV
 
 
   
405LSRS
 
 
 
 
405LSRSQ
 
   
405LSRVS
 
 
 
405LSRVSQ
 
 

* 96-well washing available with optional manifold PN 1170010, sold separately
** With available accessory module sold separately

 

405™ LS Microplate Washer Configurations
  Feature Design
HT Faster 384-well microplate washing
V Automated Internal 4-Buffer Switching  
S Ultrasonic Advantage™
  • Built-in ultrasonic cleaner for manifold self-maintenace
U 96-/384-well microplate washing
  • 96-tube (8 x 12) manifold
  • Dual-Action™
R 96-well microplate washing
  • 96-tube (8 x 12) manifold
Q Verify™ Technology
  • Built-in clog detection for dispense and aspirate tubes

 

 

General
Microplate Types 96- and 384-well
Low profile and standard height
Solid and filter bottom (option)
Onboard Software Create, edit or run multiple protocols
Software (pc control) LHC2 pc software
LHC2 Secure for 21 CFR Part 11 compliance (option)
SiLA Compliant driver (option)
Separation Biomagnetic separation, vacuum filtration (optional)
Shaking Programmable up to 60 minutes
Slow, medium, fast or variable
Soaking Programmable up to 60 minutes
Ultrasonic Advantage™ Yes (option)
Verify™ clog detection Yes (option)
Automation BioStack and 3rd party automation compatible
BioSpa 8 Automated Incubator compatible
Washing
Manifold Types 96-well washing: 96-tube manifold - straight dispense tubes
96- and 384-well washing: 96-tube Dual-Action manifold - 20° angled dispense tubes
384-well washing (fast): 192-tube Dual-Action manifold - 7° angled dispense tubes
Volume Range 25 to 3,000 µL/well, in 1 µL increments
Wash Cycles 1 - 250
Buffer/reagent selection Auto switching (internal) for up to 4 buffers (option)
Supply Bottle 4 L or 10 L (optional)
Dispense Precision <3% CV: 300 µL/well (96-well washing)
<4% CV: 80 µL/well (384-well washing)
Residual Volume <2 µL/well (96- & 384-well plates)
96-tube manifold for 96 wells; 192-tube for 384 wells
Wash Speed 96-wells, 300 µL/well, 3 cycles; <30 seconds
384-wells,100 µL/well, 3 cycles: <80 seconds
384-wells, 400 µL/well, 1 cycle: <20 seconds
Flow Rates High flow to low flow
Optimized rates for cell assays
Sterilization Chemical
Vacuum Filtration Selectable: Approximate final at 30 seconds:
Lowest: -38 mmHg
Low: -113 mmHg
Medium: -200 mmHg
High: -390 mmHg
Highest: -506 mmHg

Vacuum filtration time range: 5 to 999 seconds
Physical Characteristics
Power 100 - 240 Volts AC. 50/60 Hz
Dimensions 14" W x 17" D x 10" H
(35.6 x 43.2 x 25.4 cm)
Weight With internal buffer switching - 36 lbs (16.5 kg)
Without - 30 lbs (13.5 kg)
Regulatory
Regulatory CE and TUV marked. ROHS compliant. In Vitro Diagnostic use models are available.

Specifications are subject to change. Performance values represent the average observed factory test values.

Optional

BioSpa 8 Automated Incubator

BioSpa 8 Automated Incubator

BioSpa 8 is an automated incubator linking BioTek readers or imagers together with washers and dispensers for full workflow automation of up to 8 microplates. Temperature, CO2/O2 and humidity levels are controlled and monitored through the BioSpa software to maintain an ideal environment for cell cultures during all experimental stages.


BioStack Microplate Stacker

BioStack Microplate Stacker

BioStack™ is a compact and versatile microplate stacker compatible with BioTek’s washers, dispensers, detectors and imaging systems. BioStack offers rapid plate exchange speeds to increase throughput and enhance productivity, accommodating assay workflows both with and without plate lids.


Liquid Handling Control™ Software

Liquid Handling Control™ Software

Software is a powerful yet flexible interface for use with BioTek’s liquid handlers including the 405™ Microplate Washer, the ELx405™ Select Deep Well Washer, the EL406™ Washer Dispenser and the MultiFlo™ Dispenser. New LHC Secure offers compliance to the FDA directive 21 CFR Part 11 or whenever additional security is required.


Standard (Included)

Operators Manual

MANUAL Operator's manuals can be obtained via the Customer Resource Center

Optional

BioStack Integration Kits

7310040 BioStack Integration Kit for 405™ TS and 405™ LS

Bottles & Tubing

1173000 Dispense Bottle - with Filter, 10L accessory has picture
1173002 Cap for Dispense Bottle - with Filter, 10L
1173003 Cap for Dispense Bottle - with Filter, 4L accessory has picture
1173031 Dispense Bottle – with Filter, 4L accessory has picture
7100531 Cap for Waste Bottle, 4L (Also fits 10L or 20L) accessory has picture
7100533 Waste Tubing Set accessory has picture
7100534 Waste Bottle, 4L accessory has picture
7100537 Dispense Tubing Set – 4 Buffers
7100538 Dispense Tubing Set –1 Buffer accessory has picture
7100542 Waste Bottle with Level Sensing, 4L accessory has picture
7100543S Waste Set with Level Sensing, 4L Bottles accessory has picture
7100544 Cap for Waste Bottle with Level Sensing, 4L accessory has picture
7100556 Waste Bottle and Tubing, 20L accessory has picture
7100557 Waste Bottle and Tubing, 10L accessory has picture

Cables

75110 Serial cable, 9-pin male to 9-pin male

Dispense/Waste Systems

1170528S Complete Dispense System, 10L Bottles accessory has picture
1170529S Complete Dispense System, 4L Bottles accessory has picture
1170530 Complete Dispense/Waste System 115V/230V, 4L Bottles accessory has picture
1170531S Complete High Flow Waste System 115V/230V, 10L Bottles
1170532 Complete Dispense/High Flow Waste System 115V/230V, 4L Bottles accessory has picture
1170533 Complete Dispense/High Flow Waste System 115V/230V, 10L Bottles
1170534S Complete Waste System 115V/230V, 10L Bottles
1170535 Complete Dispense/Waste System 115V/230V, 10L Bottles
1170554 Complete Dispense/Waste System 115V/230V, 20 L Bottles
1170555S Complete Waste System 115V/230V, 20L bottles
1170556 Complete Dispense/High Flow Waste System 115V/230V, 20L Bottles
1170557S Complete High Flow Waste System 115V/230V, 20L Bottles
7100582S Vacuum Set with Level Sense Assembly, 10L accessory has picture
7100746S Complete Waste System 115V/230V, 4L Bottles accessory has picture
7100753S Complete High Flow Waste System 115V/230V, 4L Bottles
7100754 High Flow Vacuum Pump 115/230V
7103034 Standard Vacuum Pump 115V/230V accessory has picture

Filters

01310 Filter for Dispense Bottle
48294 Filter for Waste Tubing accessory has picture

Manifolds

1170010 96-Tube Dual-Action Manifold Kit, with 7° dispense tubes and tube-in-tube aspiration manifold kit for 405 HT TS and 405 HT LS models only
1172046S 96-tube Dual-Action™ Tube-in-Tube Aspiration Manifold accessory has picture
7102068S 96-tube Dual-Action™ 7° Dispense Manifold

Miscellaneous

01113 Silencing Muffler for Standard Vacuum Pump
1170008 96-well Vacuum Filtration Kit accessory has picture
1170009 96-/384-well Vacuum Filtration Kit accessory has picture
1170013 15' extension kit for use with robotic system integrations of 405 TS / LS
4030551 Vacuum Gauge/Regulator
7100025S Stylus Kit for any 405™/ELx405™ Select Deep Well manifold accessory has picture
7102215 384-well Ring Magnet accessory has picture
7102216 96-well Ring Magnet accessory has picture
7103016 96-well Flat Magnet accessory has picture
7103017 384-well Flat Magnet accessory has picture
7210515 3-Instrument Rack – for third party robotic system integration accessory has picture

Product Qualification Packages

1170543 405 LS Qualification Package - IQ/OQ/PQ

Test Plates & Reagents

1240001 Replacement 405 Verify test plate kit accessory has picture

Waste Systems

1170555S Complete Waste System 115V/230V, 20L Bottles
7100764S Waste set with Level Sensing, 20L Bottles

ONE COMPANY, ONE SOLUTION Since 2000

E-Mail: ceo@farayand.com
تلفن:72- 88334462 021
دورنگار: 88002310 021
آدرس: تهران، ميدان فاطمی، میدان گلها، خيابان كاج جنوبي،كوچه هشتم، پلاک 27
کد پستی: 1413874873
صندوق پستی: 14155/4415

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محصولات اخیر

  • Microplate Reader
  • Autoclave
  • santrifuj
  • Microplate Washer
  • Incubators
  •  PCR

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