| General |
| Microplate types |
6- to 1536-well plates |
| Other labware supported |
Microscope slides, Petri and cell culture dishes, cell culture flasks (T25, T75), counting chambers (hemocytometers), chamber slides
Support for labware up to 1.5" tall |
| Temperature control |
Incubation to 40 °C with optional environmental control cover |
| Software |
Gen5 Microplate Reader and Imager Software included
Gen5 Image and Image+ Software available for advanced image analysis (option) |
| CO2 and O2 control (option) |
0 - 20% CO2 control and 1 - 19% O2 control, with optional Gas Controller |
| Environmental control cover |
Top cover for light tight imaging and incubation control (option) |
| X/Y Stage resolution |
Lead screw driven stage with 0.1 micron resolution |
| Humidity control |
Humidity chamber with rapid gas recharge (option) |
| Imaging System |
| Imaging mode |
Fluorescence, brightfield, color brightfield, and phase contrast |
| Imaging method |
Single color, multi-color, montage, time lapse, Z-stacking, burst mode |
| Light source |
High power LEDs (available wavelengths: 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm) |
| Camera |
16-bit gray scale, Sony CCD, 1.25 megapixel |
| Camera binning |
Optional 2x2 binning for focus and/or image capture |
| Camera exposure range |
5 milliseconds to 4 seconds |
| Image outputs available |
Raw Images: 16-bit TIFF
Saved Images: TIF, JPG, BMP, PNG, EMF, GIF
Movies: MP4, WMV |
| Objective capacity |
6 onboard, user-replaceable objectives |
| Objectives available |
Fluorescence:
Air: 1.25x, NA .04; 2.5x (2.25x eff), NA: .07; 2.5x (2.75x eff), NA: .12; 4x, NA: .13; 10x, NA: .30;
20x, NA: .45; 40x, NA.60; 60x, NA .70
Oil: 60x, NA:1.42; 100x, NA: 1.40
Phase objectives available: 4x, NA: .13; 10x, NA: .30; 20x, NA: .45; 40x, NA .60 |
| Image filter cube capacity |
4 user-replaceable fluorescence cubes plus brightfield channel |
| Imaging filter cubes available |
DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7, Acridine Orange, CFP-YFP FRET, Chlorophyll, Phycoerythrin (PE), Propidium Iodide, CY5.5, TagBFP, GFP (Ex)-CY6 (Em), RFP (Ex)-CY5 (Em), Alexa 568, Ex377 / Em647 |
| Automated functions |
Autofocus, user-trained autofocus, auto exposure, auto-LED intensity |
| Autofocus method |
Image-based autofocus
Laser autofocus option |
| Image collection rate |
Single well fastest frame rate capture:
*Full Resolution: up to 10 frames per second for single color images
*2x2 Binning: up to 20 frames per second for single color images |
| Microscope stage control |
Gen5 Software Control
Optional joystick controller |
| Reagent Injectors (option) |
| Number |
2 syringe pumps |
| Supported labware |
6- to 384-well microplates, Petri and cell culture dishes, chamber slides |
| Dead volume |
<1.65 mL with back flush |
| Dispense tip options |
Aligned tip - aligned with optical path for dispensing for fast kinetic assays
Offset tip - dispensing is offset from the optical path |
| Dispense volume |
5 - 1000 µL in 1 µL increments |
| Dispense accuracy |
+1 µL or 2% |
| Dispense precision |
<2% at 50 - 200 µL |
| Physical Characteristics |
| Power |
250 Watts max. |
| Dimensions |
With cover closed or without cover: 18.3" D, 17.9" W, 14.1" H (46.5 cm x 45.5 cm x 35.8 cm)
With cover fully open: 18.3" D, 17.9" W, 27.5" H (46.5 cm x 45.5 cm x 69.8 cm) |
| Weight |
Without environmental control cover: 51 lbs (23.1 kg)
With environmental control cover: 58 lbs (26.3 kg) |
| Regulatory |
| Regulatory |
CE and TUV marked. RoHS compliant. Models for In Vitro Diagnostic use are available. |